Mega Base Rapid Agarose

£26.22£442.37

Our very high gel strength Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses.

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Description

Our very high gel strength Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic, added to the very low sulfate content, produces a strong intercatenary interaction, yielding a gel with very high gel strength and higher exclusion limit. Typically used in

Applications

  • Conventional Electrophoresis: can be used in a wide range of concentrations.
  • Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.
  • Blotting.
  • Agarose Beads preparation
  • Cell and enzyme immobilization.

Features

  • Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes but also with large sized particles like viruses and ribosomes.
  • High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used.
  • Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
  • Greater thermal stability due to high hysteresis (difference, between gelling and melting temperatures).
  • Exceptionally low absorption of staining agents.
  • The absence of toxicity (the alternative is polyacrylamide which can be toxic)

Additional information

Size

25g, 100g, 500g

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