Description
Our very high gel strength Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic, added to the very low sulfate content, produces a strong intercatenary interaction, yielding a gel with very high gel strength and higher exclusion limit. Typically used in
Applications
- Conventional Electrophoresis: can be used in a wide range of concentrations.
- Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.
- Blotting.
- Agarose Beads preparation
- Cell and enzyme immobilization.
Features
- Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes but also with large sized particles like viruses and ribosomes.
- High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis (difference, between gelling and melting temperatures).
- Exceptionally low absorption of staining agents.
- The absence of toxicity (the alternative is polyacrylamide which can be toxic)
Reviews
There are no reviews yet.