Alkaline Peptone Water, dehydrated culture medium Cat Nº: AG-1407
For the enrichment of Vibrio species from food, water and clinical samples
Formula in g/l
|Final pH 8.6 ± 0.2 at 25ºC|
Suspend 25 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. To make a 10x-strength base suspend 250 grams instead of 25 grams. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. The prepared medium should be stored at 2-8ºC. The color is amber. The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.
ALKALINE PEPTONE WATER is used for the enrichment of Vibrio cholera and Vibrio species from food, water, feces and clinical studies.This medium has been recommended by various authors to increase the recovery of Vibrio species in fecal materials and other samples. Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance and encourages the growth of Vibrio cholerae.It is claimed that raising the medium’s pH leads the medium’s alkalinity to inhibit most of the unwanted flora background, leaving the viability of the Vibrio species intact.
Growth in tubes is indicated by turbidity compared to an uninoculated control. Additional steps are recommended, like plating onto a selective and non-selective media for isolation and morphology, and biochemical and serological studies for identification
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 37ºC ± 2ºC and observed after 24 hours.
Escherichia coli ATCC 25922
Streptococcus pneumoniae ATCC 6301
Vibrio cholerae EITOR ATCC 14033
Colwell, R.R. 1996. Global climate and infectious disease: the cholera paradigm. Science 274. Kelly, M,T, F.W. Hickman-Bremer, and J.J. Framer III
Once opened keep powdered medium closed to avoid hydration.
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