Acetamide Broth


Acetamide Broth

100 in stock

SKU: 1211-500gm Category:


ACETAMIDE BROTH CAT  Nº:  AG-1211, Dehydrated culture Media for Microbiology

For the differentiation of non-fermentative gram negative bacteria, in particular, Pseudomonas  aeruginosa

Formula in g/l

Sodium Chloride


Monopotassium Phosphate 




Phenol Red


Dipotassium Phosphate 






Final pH7.0 ± 0.2 at 25ºC


Suspend 17.2 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution Sterilize by filtration. AVOID OVERHEATING. DO NOT AUTOCLAVE. Dispense into appropriate containers. The prepared medium should be stored at 2-8ºC. The color is pink- orange.The dehydrated medium should be homogeneous, free-flowing and pink in color. If there are any physical changes, discard the medium.

Caution: This medium contains acetamide. It is toxic if swallowed, inhaled or comes into contact with skin. Wear gloves and eye/face protection.


ACETAMIDE AGAR is used to determine the ability of non-fermenting Gram-negative bacteria to deaminate the acetamide. The deamination of the acetamide produces ammonia which increases the pH of the medium. The resulting alkalinisation is shown by a color change of the Phenol Red from yellow-orange to purple-red.Acetamide deamination is accomplished by Pseudomonas.aeruginosa, Pseudomonas acidovorans, Group III (Achromobacter xylosoxidans), and Alcaligenes odorans.Acetamide is a carbon source. Dextrose is a fermentable carbohydrate providing carbon and energy, the Potassium salts have a high buffering capacity. Sodium chloride supplies essential electrolytes for transport and osmotic balance, Phenol red is a pH indicator and Bacteriological agar is the solidifying agent.A positive reaction turns the medium an intense purple-red. P. aeruginosa is confirmed by positive  asparagine  and acetamide  tests.Pseudomonas aeruginosa is an opportunist pathogen for humans, capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water are Pseudomonas aeruginosa free at the time of their commercialization. This microorganism can also be found in swimming pool water.

Microbiological Test

The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2ºC and observed after 24-48 hours                                                                                  


 Microorganisms    Growth Purple – Red  

Escherichia coli ATCC 25922


Proteus mirabilis ATCC 29906



Pseudomonas aeruginosa ATCC 9027


 PseudomonasaeruginosaATCC25668 Good      


Gilardi. 1974. Antonie van Leewenhoek. J. Microbiol. Serol. 39:229. Buhlmann, Vischer and Bruhin. 1961. J. Bacteriol. 82:787.

Clesceri, Greenberg and Eaton (ed.) 1998. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.

Murray, Baron, Pfaller, Tenover and Yolken (ed.). 1999. Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C


Once opened keep powdered medium closed to avoid hydration.

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Additional information

Weight0.5 kg